(1) any situation in which the program cannot proceed safely, (2) allergic constitution, (3) serious basic diseases affecting survival, including uncontrolled malignant tumors with multiple metastases that cannot be resected, blood diseases, malignant fluid, active bleeding, severe malnutrition, HIV, etc., The main organ function is abnormal (4) liver function was obviously abnormal: ALT/AST increased by more than 5 times the normal upper limit, increase of total bilirubin more than 2 times of normal upper limit, (5) obstructive pneumonia, severe pulmonary fibrosis, alveolar proteinosis and allergic alveolitis caused by lung tumors, (6) pregnancy: positive pregnancy test for women of child-bearing age, (7) the patient may be transferred to a non-participating hospital within 72h, (8) continuous use of immunosuppressants or organ transplants within the past 6 months, (9) in vitro life support patients (ECMO, ECCO2R, RRT), (10) expected survival no more than 48 hours.

(1) any situation in which the program cannot proceed safely, (2) allergic constitution, (3) serious basic diseases affecting survival, including uncontrolled malignant tumors with multiple metastases that cannot be resected, blood diseases, malignant fluid, active bleeding, severe malnutrition, HIV, etc., The main organ function is abnormal (4) liver function was obviously abnormal: ALT/AST increased by more than 5 times the normal upper limit, increase of total bilirubin more than 2 times of normal upper limit, (5) obstructive pneumonia, severe pulmonary fibrosis, alveolar proteinosis and allergic alveolitis caused by lung tumors, (6) pregnancy: positive pregnancy test for women of child-bearing age, (7) the patient may be transferred to a non-participating hospital within 72h, (8) continuous use of immunosuppressants or organ transplants within the past 6 months, (9) in vitro life support patients (ECMO, ECCO2R, RRT), (10) expected survival no more than 48 hours.