* exclusion criteria included males or females diagnosed with any chronic immune problems, including autoimmune diseases, chronic or severe infections. * pregnant, breastfeeding, and females using hormonal contraceptives were also excluded.

* exclusion criteria included males or females diagnosed with any chronic immune problems, including autoimmune diseases, chronic or severe infections. * pregnant, breastfeeding, and females using hormonal contraceptives were also excluded.

- exclusion criteria included males or females diagnosed with any chronic immune problems, including autoimmune diseases, chronic or severe infections. - pregnant, breastfeeding, and females using hormonal contraceptives were also excluded.

- exclusion criteria included males or females diagnosed with any chronic immune problems, including autoimmune diseases, chronic or severe infections. - pregnant, breastfeeding, and females using hormonal contraceptives were also excluded.