Pregnancy and breastfeeding G6PDH deficiency Severe renal insufficiency (glomerular filtration rate <30 mL/min/1.73m2) Active chronic hepatitis Severe hepatic disease defined by GOT or GPT levels three times above the normal upper limit Patients with history of allergic reaction or significant sensitivity to methylene blue Treatment with immunosuppressive agents Use of other investigational drugs in the moment of inclusion

Pregnancy and breastfeeding G6PDH deficiency Severe renal insufficiency (glomerular filtration rate <30 mL/min/1.73m2) Active chronic hepatitis Severe hepatic disease defined by GOT or GPT levels three times above the normal upper limit Patients with history of allergic reaction or significant sensitivity to methylene blue Treatment with immunosuppressive agents Use of other investigational drugs in the moment of inclusion